1224 .
ECTOTHIORHODOSPIRA VARIABILIS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 50.0 | g | ||
|
|
Na2CO3 | 5.0 | g | ||
|
|
NaHCO3 | 15.0 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
K2HPO4 | 0.5 | g | ||
|
|
MgCl2 x 6 H2O | 0.2 | g | ||
|
|
NH4Cl | 0.5 | g | ||
|
|
Na2S x 9 H2O | 0.5 | g | ||
|
|
Na2S2O3 x 5 H2O | 0.5 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
Na acetate | 0.5 | g | ||
|
|
Trace element solution SL-10 | 1.0 | ml | ||
|
|
Vitamin B12 | 20.0 | µg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Final pH 9.0 - 9.5 | |||||
| 2 Prepare and boil the medium in the absence of Na2S x 9 H2O, NaHCO3, Vitamin B12, CaCl2 x 2 H2O, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilised) and Na2S2O3 x 5 H2O from sterile stock solutions. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||