1245 .
OTEB-MEDIUM
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* Medium for Treponema pedis DSM 18691
Plus 10% fetal bovine serum; anaerobic (85% N2; 10% H2; 5% CO2)
Growth conditions: 37 °C
Equipment needed:
Final pH:
6.8 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Proteose peptone no. 2 | 5.0 | g | ||
|
|
Heart Infusion Broth | 5.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
Potassium Phosphate , dibasic | 2.0 | g | ||
|
|
Sodium Chloride | 5.0 | g | ||
|
|
Magnesium sulfate | 0.1 | g | ||
|
|
Acetic acid | 1.6 | ml | ||
|
|
Propionic acid | 0.6 | ml | ||
|
|
n-Butyric acid | 0.4 | ml | ||
|
|
n-Valeric acid | 0.1 | ml | ||
|
|
Isovaleric acid | 0.1 | ml | ||
|
|
Isobutyric acid | 0.1 | ml | ||
|
|
2-Methylbutyric acid | 0.1 | ml | ||
|
|
Vitamin K1
(1% soln) |
0.1 | ml | ||
|
|
L-Cysteine | 0.5 | g | ||
|
|
Fetal bovine serum , inactivated | 100.0 | ml | ||
|
|
Sugar Stock Solution | 100.0 | ml | ||
|
|
Fetal bovine serum | 100.0 | g/l | ||
|
|
Distilled water | 800.0 | ml | ||
| 1 OTEB-medium is available from Anaerobe Systems (http://www.anaerobesystems.com) | |||||
| 2 The following text was taken from the above page: - ORAL TREPONEME ENRICHMENT BROTH (OTEB) - Products: AS-603 Oral Treponeme Enrichment Broth (OTEB) 10 tubes / pkg | |||||
| 3 Intended Use: OTEB is an enriched medium for the isolation, cultivation and maintenance of Treponemes found as part of the oral flora. | |||||
| 4 OTEB is a complex peptone-yeast extract medium containing volatile fatty acids and serum for a source of long chain fatty acids. Volatile fatty acids and serum are necessary nutrients for recovery of Treponemes from the oral flora. Inoculation of the medium can be done using a gassing cannula or inside an anaerobic chamber. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. | |||||
| 5 Final pH 7.0 +/- 0.2 at 25 degrees C. | |||||
| 6 Final volume 7.0 ml +/- 0.7. | |||||
Sugar Stock Solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Glucose | 8 | g |
|
|
Fructose | 8 | g |
|
|
Sucrose | 8 | g |
|
|
Maltose | 8 | g |
|
|
Maltose | 8 | g |
|
|
Ribose | 8 | g |
|
|
Xylose | 8 | g |
|
|
Pectin | 8 | g |
|
|
Starch
(soluble) |
8 | g |
|
|
Sodium pyruvate | 8 | g |
|
|
Thiamine pyrophosphate | 750 | µg |
|
|
Distilled water | 1000 | ml |