1264 .
SPIROCHAETA SP. MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.4
Description:
Not in use anymore!
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 30.0 | g | ||
|
|
NH4Cl | 0.3 | g | ||
|
|
CaCl2 x 2 H2O | 0.3 | g | ||
|
|
MgCl2 x 2 H2O | 3.0 | g | ||
|
|
Starch
(soluble) |
5.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Peptone
(Difco) |
5.0 | g | ||
|
|
Phosphate buffer
(10%, pH 7.4) |
10.0 | ml | ||
|
|
Trace elements (Pfennig) | 1.0 | ml | ||
|
|
Resazurin | 0.5 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Boil medium and cool under N2 stream on ice. Check pH (7.4). Autoclave aliquots and add the following sterile solutions (per 10 ml): | |||||
|
|
Na2S
(3%) |
0.1 | ml | ||
|
|
Vitamine B12
(1 mg/10 ml) |
50.0 | µl | ||
Trace elements (Pfennig) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 500 | mg |
|
|
FeSO4 x 7 H2O | 200 | mg |
|
|
ZnSO4 x 7 H2O | 10 | mg |
|
|
H3BO3 | 30 | mg |
|
|
CoCl2 x 6 H2O | 20 | mg |
|
|
NiCl2 x 6 H2O | 2 | mg |
|
|
Na2MoO4 x 2 H2O | 3 | mg |
|
|
MnCl2 x 4 H2O | 3 | mg |
|
|
CuCl2 x 2 H2O | 1 | mg |
|
|
Distilled water | 1000 | ml |