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DSMZ

DSMZ 1266a . MEDIUM GY VL55

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Equipment needed:
Final pH: 5.5
Select a strain (optional):

ml
Compound Amount Unit
Solution 1 500 ml
Solution 2 500 ml
1 After autoclaving, cool to 50-60°C and combine the solution by pouring solution 2 to solution 1.

Solution 1 #

492
Compound Amount Unit
MES
(2-Morpholinoethanesulfonic acid) 		1.95 g
20 mM MgSO4 x 7 H2O 10.00 ml
30 mM CaCl2 x 2 H2O 10.00 ml
20mM (NH4)2HPO4 10.00 ml
Selenite-tungstate solution 1.00 ml
Trace element solution SL-10 1.00 ml
Distilled water 460.00 ml
1 Adjust to pH 5.5 with NaOH/KOH solution (see below).

Solution 2 #

500
Compound Amount Unit
Glucose 1.0 g
Yeast extract 0.5 g
Distilled water 500.0 ml
Agar , for solid medium 20.0 g

20 mM MgSO4 x 7 H2O #

100
Compound Amount Unit
H2O 100.00 ml
MgSO4 x 7 H2O 0.49 g

30 mM CaCl2 x 2 H2O #

100
Compound Amount Unit
H2O 100.00 ml
CaCl2 x 2 H2O 0.44 g

20mM (NH4)2HPO4 #

100
Compound Amount Unit
H2O 100.00 ml
(NH4)2HPO4 0.26 g

NaOH/KOH #

100
Compound Amount Unit
H2O 100.00 ml
NaOH 0.80 g
KOH 0.56 g

Selenite-tungstate solution #

1000
Compound Amount Unit
NaOH 0.5 g
Na2SeO3 x 5 H2O 3.0 mg
Na2WO4 x 2 H2O 4.0 mg
Distilled water 1000.0 ml

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%) 		10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.