1275 .
GEORGFUCHSIA MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 10.00 | ml | ||
|
|
Solution B | 0.50 | ml | ||
|
|
Solution C | 0.50 | ml | ||
|
|
Solution D | 0.25 | ml | ||
|
|
Solution E | 0.40 | ml | ||
| 1 Solutions are prepared anaerobically under N2:CO2. Solutions A, B, C and D are autoclaved. After cooling, the solutions are combined under N2 gas. To 10 ml solution A, add 0.5 ml solution B, 0.5 ml solution C, 0.25 ml solution D and 0.4 ml solution E. | |||||
| 2 pH should be 7.3 without further adjustment. Add new toluene solution to the cultures about every 7 days. | |||||
Solution A #
905
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 0.65 | g |
|
|
Na2HPO4 | 0.20 | g |
|
|
Resazurin | 0.50 | mg |
|
|
Trace element solution SL-10 | 1.00 | ml |
|
|
Selenite-tungstate solution | 4.00 | ml |
|
|
Distilled water | 900.00 | ml |
Solution B #
50
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CaCl2 | 0.11 | g | ||
|
|
MgCl2 | 0.10 | g | ||
|
|
Distilled water | 50.00 | ml | ||
|
|
Vitamin solution | 1.00 | ml | ||
| 1 Add 1 ml vitamin solution (see below) after autoclaving and cooling. | |||||
Solution C #
50
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4HCO3 | 0.44 | g |
|
|
NaHCO3 | 3.73 | g |
|
|
L-Cysteine HCl x H2O | 0.03 | g |
|
|
Distilled water | 50.00 | ml |
Solution E #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Toluene | 58 | µl | ||
|
|
Distilled water | 100 | ml | ||
| 1 Sterile distilled water gassed with dinitrogen, 100.00 ml. Add 58 µl toluene. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg |
|
|
Folic acid | 20 | mg |
|
|
Pyridoxine hydrochloride | 100 | mg |
|
|
Thiamine-HCl x 2 H2O | 50 | mg |
|
|
Riboflavin | 50 | mg |
|
|
Nicotinic acid | 50 | mg |
|
|
D-Calcium pantothenate | 50 | mg |
|
|
Vitamin B12 | 1 | mg |
|
|
p-Aminobenzoic acid | 50 | mg |
|
|
Lipoic acid | 50 | mg |
|
|
Distilled water | 1000 | ml |