1369 .
ECTOTHIORHODOSPIRA MAGNA MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.5 | g | ||
|
|
NaCl | 30.0 | g | ||
|
|
NH4Cl | 0.5 | g | ||
|
|
MgCl2 x 6 H2O | 0.2 | g | ||
|
|
Na-acetate | 1.0 | g | ||
|
|
Na2S2O3 | 0.5 | g | ||
|
|
Na2S x 9 H2O | 0.5 | g | ||
|
|
NaHCO3 | 5.0 | g | ||
|
|
Na2CO3 | 5.0 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
Vitamin B12 | 10.0 | µg | ||
|
|
Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 pH 9.0-9.5 | |||||
| 2 Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere. | |||||
| 3 Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S x 9 H2O, Na2CO3 from sterile stock solutions. The final pH of the medium should be 9.0-9.5. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.03 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||