1457 .
ALCALILIMNICOLA EHRLICHII MHLE-1 MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
9.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 496 | ml | ||
|
|
Solution B | 500 | ml | ||
|
|
Solution C | 10 | ml | ||
|
|
Agar , for solid medium | 20 | g | ||
| 1 Autoclave solution A without the vitamin solution. Autoclave solution B in half full, closed bottles. Autoclave solution C. Combine the sterile solutions A, B, and C after cooling. Aseptically add 1.0 ml of the vitamin solution (filter sterilized) to 1 litre of the sterile medium. | |||||
| 2 Adjust to pH 9.8. The medium may be solidified by adding 20 g/l agar, but care should be taken that the temperature of the solutions to be mixed are about 50°C, otherwise the agar will darken and degrade. | |||||
Solution A #
496
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 0.10 | g |
|
|
MgSO4 | 25.00 | mg |
|
|
NaCl | 60.00 | g |
|
|
K2HPO4 | 0.15 | g |
|
|
KH2PO4 | 0.08 | g |
|
|
Trace element solution SL-10 | 5.00 | ml |
|
|
Tungstate
(0.1 mM) |
0.30 | ml |
|
|
Vitamin solution | 1.00 | ml |
|
|
Distilled water | 490.00 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
D-Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%; 7.7 M) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Make up to a final volume of 1000.0 ml. | |||||