1486 .
GBS salts modified for Thermocrinis jamiesonii
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
7.2 - 7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaH2PO4 | 1.380 | g | ||
|
|
NaCl | 3.000 | g | ||
|
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NH4Cl | 0.107 | g | ||
|
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CaCl2 x 2 H2O | 0.015 | g | ||
|
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MgSO4 x 7 H2O | 0.123 | g | ||
|
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KCl | 0.150 | g | ||
|
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Na2SO4 | 0.300 | g | ||
|
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Mineral solution | 5.000 | ml | ||
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Distilled water | 1000.000 | ml | ||
| 1 Adjust pH to 7.2. | |||||
| 2 Prepare the medium under a nitrogen atmosphere. Dispense 5 ml into 25 ml tubes fitted with rubber stoppers. | |||||
| 3 Autoclave at 121 °C for 15 min. | |||||
| 4 after autoklaving, add the following from anaerobic, sterile (autoclaved) stock solutions (e.g., 100x) | |||||
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Sodium acetate trihydrate | 2.000 | mM | ||
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Sodium thiosulfate pentahydrate | 2.000 | mM | ||
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Sodium bicarbonate | 1.000 | mM | ||
| 5 Before inoculation 2% sterile O2 or 10% sterile air is added to the headspace. | |||||
Mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 3.7000 | g | ||
|
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FeSO4 x 7 H2O | 1.1000 | g | ||
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ZnSO4 x 7 H2O | 0.0288 | g | ||
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MnCl2 x 4 H2O | 0.0984 | g | ||
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H3BO3 | 0.0310 | g | ||
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CoCl2 x 6 H2O | 0.0238 | g | ||
|
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CuCl2 x 2 H2O | 0.0172 | g | ||
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Na2MoO4 x 2 H2O | 0.0242 | g | ||
|
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Distilled water | 1000.0000 | ml | ||
| 1 Adjust pH to 6.0 with KOH | |||||