1497 .
HALOARCHAEUM SALINUM MEDIUM
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Selected strain: Halarchaeum salinum DSM 27207
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Mineral Salts Solution for solid Medium , alternative | 500 | ml |
|
|
Mineral Salts Solution for liquid Medium , alternative | 500 | ml |
Mineral Salts Solution for solid Medium #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
L-Glutamic acid | 1.0 | g |
|
|
Trisodium citrate x 2 H2O | 1.0 | g |
|
|
KH2PO4 | 0.5 | g |
|
|
NH4Cl | 0.5 | g |
|
|
K2SO4 | 2.5 | g |
|
|
MgCl2 x 7 H2O | 20.0 | g |
|
|
Casamino acids | 2.0 | g |
|
|
Yeast extract
(Oxoid) |
1.0 | g |
|
|
FeSO4 | 2.0 | mg |
|
|
Trace Element Solution | 1.0 | ml |
|
|
Distilled water | 300.0 | ml |
|
|
NaCl | 100.0 | g |
|
|
Agar | 20.0 | g |
|
|
Distilled water | 200.0 | ml |
Mineral Salts Solution for liquid Medium #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
L-Glutamic acid | 1.0 | g | ||
|
|
Trisodium citrate x 2 H2O | 1.0 | g | ||
|
|
KH2PO4 | 0.5 | g | ||
|
|
NH4Cl | 0.5 | g | ||
|
|
K2SO4 | 2.5 | g | ||
|
|
MgCl2 x 7 H2O | 20.0 | g | ||
|
|
NaCl | 100.0 | g | ||
|
|
Casamino acids | 2.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
FeSO4 | 2.0 | mg | ||
|
|
Trace Element Solution | 1.0 | ml | ||
|
|
Distilled water | 500.0 | ml | ||
| 1 Adjust to pH 5.5 with 10% KOH and sterilize by autoclaving. | |||||
Trace Element Solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CoCl2 x 6 H2O | 0.30 | g | ||
|
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
|
ZnCl2 | 0.10 | g | ||
|
|
H3BO4 | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.10 | g | ||
|
|
NiCl2 x 6 H2O | 0.10 | g | ||
|
|
CuCl2 x 6 H2O | 0.01 | g | ||
|
|
AlCl3 | 0.04 | g | ||
|
|
Na2WO4 x 2 H2O | 0.02 | g | ||
|
|
CaCl2 x 2 H2O | 1.00 | g | ||
|
|
BaCl2 x 2 H2O | 0.01 | g | ||
|
|
Na2S2O3 x 5 H2O | 2.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust to pH 4.0. | |||||