DSMZ 14 . CLOSTRIDIUM FORMICOACETICUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Clostridium formicaceticum DSM 92

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 5.00 g 4.946 -
K2HPO4 10.00 g 9.891 56.789
Trace element solution SL-4 10.00 ml - -
Pyridoxine hydrochloride
(0.1% w/v)
1.00 ml 9.9e-4 0.005
Na-thioglycolate 0.75 g 0.742 6.502
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
Na2CO3 1.00 g 0.989 9.332
D-Fructose 5.00 g 4.946 27.451
Agar , for solid medium
optional
15.00 g 14.837 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate and fructose) and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 8.0.
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 8
Clostridium formicaceticum DSM 92

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional
14.837 -
K2HPO4 9.891 56.789
D-Fructose 4.946 27.451
Yeast extract 4.946 -
Na2CO3 0.989 9.332
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen anaerobe 8 strains
temperature mesophilic 8 strains