1521 .
ARCOBACTER ANAEROPHILUS MEDIUM
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
NaCl | 20.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
Sodium citrate | 0.30 | g | ||
|
|
Yeast extract | 0.50 | g | ||
|
|
Ammonium acetate | 1.00 | g | ||
|
|
Lactate | 1.00 | g | ||
|
|
Pyruvate | 1.00 | g | ||
|
|
SL10 | 1.00 | ml | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add distilled water to 1 liter, boil the medium and cool under N2, distribute under N2 and autoclave. After autoclaving the following sterilized solutions are added: | |||||
|
|
NaHCO3
(10%) |
10.00 | ml | ||
|
|
Na2S x 9 H2O
(1 M) |
10.00 | ml | ||
|
|
Vitamin B12 | 10.00 | ml | ||