1538 .
ANAEROBACTERIUM MEDIUM
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Final pH:
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ml
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 0.20 | g |
|
|
NH4Cl | 0.25 | g |
|
|
NaCl | 1.00 | g |
|
|
MgCl2 x 6 H2O | 0.40 | g |
|
|
KCl | 0.50 | g |
|
|
CaCl2 x 2 H2O | 0.15 | g |
|
|
Yeast extract | 1.00 | g |
|
|
Cellobiose | 10.00 | g |
|
|
NaHCO3 | 2.52 | g |
|
|
Na2S x 9 H2O | 0.36 | g |
|
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Trace element solution SL-10 | 1.00 | ml |
|
|
Vitamin solution | 1.00 | ml |
|
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Selenite-tungstate solution | 1.00 | ml |
|
|
Distilled water | 1000.00 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg/l |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Vitamin B12 | 50 | mg |
|
|
Distilled water | 1000 | ml |
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
|
Distilled water | 1000.0 | ml |