1633 .
V2 MEDIUM
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Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone
(Bacto) |
0.250 | g | ||
|
|
Yeast extract
(Bacto) |
0.250 | g | ||
|
|
HEPES | 2.380 | g | ||
|
|
Mineral salts solution | 20.000 | ml | ||
|
|
Trace element solution | 1.000 | ml | ||
|
|
NaHCO3 | 0.025 | g | ||
|
|
CaCO3 | 0.025 | g | ||
|
|
CaCl2 x 2 H2O | 0.015 | g | ||
|
|
MgSO4 x 7 H2O | 0.049 | g | ||
|
|
KNO3 | 0.010 | g | ||
|
|
KH2PO4 | 1.400 | mg | ||
|
|
NH4Cl | 0.530 | mg | ||
|
|
Agar
(Bacto) |
15.000 | g | ||
|
|
Double distilled water | 730.000 | ml | ||
| 1 Sterilize 20 min./121°C. After cooling to 60°C add: | |||||
|
|
N-Acetylglucosamine
(10% stock solution, 10 % stock solution) |
8.000 | ml | ||
|
|
Glucose
(2.5%, sterile-filtered) |
10.000 | ml | ||
|
|
Vitamin solution (double conc.)
(medium 621) |
5.000 | ml | ||
| 2 Adjust pH to 7.0 with 40% KOH. | |||||
Mineral salts solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | ||
|
|
MgSO4 x 7 H2O | 29.70 | g | ||
|
|
CaCl2 x 2 H2O | 3.34 | g | ||
|
|
Na2MoO7O4 x 2 H2O | 12.67 | mg | ||
|
|
FeSO4 x 7 H2O | 99.00 | mg | ||
|
|
"Metals 44" | 50.00 | ml | ||
|
|
Distilled water | 950.00 | ml | ||
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||
"Metals 44" #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na-EDTA | 250.0 | mg |
|
|
ZnSO4 x 7 H2O | 1095.0 | mg |
|
|
FeSO4 x 7 H2O | 500.0 | mg |
|
|
MnSO4 x H2O | 154.0 | mg |
|
|
CuSO4 x 5 H2O | 39.2 | mg |
|
|
Co(NO3)2 x 6 H2O | 24.8 | mg |
|
|
Na2B4O7 x 10 H2O | 17.7 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na-Nitrilotriacetat | 1.50 | g |
|
|
MnSO4 x H2O | 0.50 | g |
|
|
Fe SO4 x 7 H2O | 0.10 | g |
|
|
Co(NO3)2 x 6 H2O | 0.10 | g |
|
|
ZnCl2 | 0.10 | g |
|
|
NiCl2 x 6 H2O | 0.05 | g |
|
|
H2SeO3 | 0.05 | g |
|
|
CuSO4 x 5 H2O | 0.01 | g |
|
|
AlK(SO4)2 x 12 H2O | 0.01 | g |
|
|
H3BO3 | 0.01 | g |
|
|
Na2MoO4 x 2 H2O | 0.01 | g |
|
|
Na2WO4 x 2 H2O | 0.01 | g |
|
|
Double distilled water | 1000.00 | ml |
Vitamin solution (double conc.) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 4.0 | mg |
|
|
Folic acid | 4.0 | mg |
|
|
Pyridoxine hydrochloride | 20.0 | mg |
|
|
Riboflavine | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 10.0 | mg |
|
|
Nicotinamide | 10.0 | mg |
|
|
D-Calcium pantothenate | 10.0 | mg |
|
|
Vitamin B12 | 0.2 | mg |
|
|
p-Aminobenzoic acid | 10.0 | mg |
|
|
Double distilled water | 1000.0 | ml |