163 .
HALODESULFOVIBRIO (POSTGATE) MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
6.8 - 7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 980 | ml | ||
|
|
Solution B | 10 | ml | ||
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Solution C | 10 | ml | ||
| 1 Bring solution A to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary. | |||||
Solution A #
980
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 25.0 | g |
|
|
K2HPO4 | 0.5 | g |
|
|
NH4Cl | 1.0 | g |
|
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Na2SO4 | 1.0 | g |
|
|
CaCl2 x 2 H2O | 0.1 | g |
|
|
MgSO4 x 7 H2O | 2.0 | g |
|
|
Na-DL-lactate | 2.0 | g |
|
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Yeast extract | 1.0 | g |
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml |
|
|
Distilled water | 980.0 | ml |
Solution C #
10
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na-thioglycolate | 0.1 | g |
|
|
Ascorbic acid | 0.1 | g |
|
|
Distilled water | 10.0 | ml |