1697 .
THIOSOCIUS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 16.00 | g | ||
|
|
Na2SO4 | 2.50 | g | ||
|
|
CaCl2 x 2 H2O | 1.00 | g | ||
|
|
KCl | 0.45 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
K2HPO4 | 0.14 | g | ||
|
|
Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | ||
|
|
HEPES buffer
(1 M, pH 8.0) |
20.00 | ml | ||
|
|
MgCl2 x 6 H2O | 6.00 | g | ||
|
|
NaHCO3 | 1.00 | g | ||
|
|
Na2S2O3 x 5 H2O | 2.50 | g | ||
|
|
Wolin's vitamin solution (10x) | 1.00 | ml | ||
|
|
Distilled water | 980.00 | ml | ||
| 1 Dissolve ingredients (except HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins), dispense medium in test tubes or Erlenmeyrer flasks to 20% of volume, close with screw caps and autoclave. Add HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 8.0. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.03 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||
Wolin's vitamin solution (10x) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg |
|
|
Folic acid | 20 | mg |
|
|
Pyridoxine hydrochloride | 100 | mg |
|
|
Thiamine HCl | 50 | mg |
|
|
Riboflavin | 50 | mg |
|
|
Nicotinic acid | 50 | mg |
|
|
Calcium D-(+)-pantothenate | 50 | mg |
|
|
Vitamin B12 | 1 | mg |
|
|
p-Aminobenzoic acid | 50 | mg |
|
|
(DL)-alpha-Lipoic acid | 50 | mg |
|
|
Distilled water | 1000 | ml |