1728 .
MMB MEDIUM (ATCC 2254)
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Final pH:
7.0 - 7.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Peptone | 0.1 | g | ||
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Yeast extract | 0.1 | g | ||
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Glucose | 1.0 | g | ||
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(NH4)2SO4 | 0.2 | g | ||
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Hutner's salts | 20.0 | ml | ||
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Vitamin solution | 10.0 | ml | ||
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Distilled water | 970.0 | ml | ||
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Agar , for solid medium | 15.0 | g | ||
| 1 pH 7.0- 7.5 | |||||
| 2 For solid medium, add 15g of agar per liter. | |||||
| 3 Glucose and vitamin solution should be filter-sterilized separately. | |||||
Hutner's salts #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | ||
|
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MgSO4 x 7 H2O | 29.700 | g | ||
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CaCl2 x 2 H2O | 3.335 | g | ||
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(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | ||
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FeSO4 x 7 H2O | 99.000 | mg | ||
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"Metals 44" | 50.000 | ml | ||
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Distilled water | 950.000 | ml | ||
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
"Metals 44" #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na-EDTA | 250.0 | mg | ||
|
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ZnSO4 x 7 H2O | 1095.0 | mg | ||
|
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FeSO4 x 7 H2O | 500.0 | mg | ||
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MnSO4 x H2O | 154.0 | mg | ||
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CuSO4 x 5 H2O | 39.2 | mg | ||
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Co(NO3)2 x 6 H2O | 24.8 | mg | ||
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Na2B4O7 x 10 H2O | 17.7 | mg | ||
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Distilled water | 1000.0 | ml | ||
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
|
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Pyridoxine hydrochloride | 10.0 | mg |
|
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
|
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D-Calcium pantothenate | 5.0 | mg |
|
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Vitamin B12 | 0.1 | mg |
|
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p-Aminobenzoic acid | 5.0 | mg |
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Lipoic acid | 5.0 | mg |
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Distilled water | 1000.0 | ml |