1734 .
THIOMICROSPIRA SP. MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 17.50 | g | ||
|
|
NH4Cl | 0.40 | g | ||
|
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
|
Trace element solution | 10.00 | ml | ||
|
|
Na2S2O3 x 5 H2O | 10.00 | g | ||
|
|
KH2PO4 | 0.14 | g | ||
|
|
NaHCO3 | 0.50 | g | ||
|
|
Seven vitamins solution | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except hydrogenphosphate, hydrogencarbonate and vitamins), adjust pH to 7.5 and autoclave. K2HPO4 is autoclaved separately. Filter sterilize the vitamins and NaHCO3 stock solutions. Adjust pH of the complete medium to 8.0 with sterile 0.4% (w/v) Na2CO3 solution, if necessary. | |||||
| 2 Note: Incubate in Erlenmeyer flasks with shaking. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 11.00 | g | ||
|
|
CaCl2 x 2 H2O | 7.34 | g | ||
|
|
MnCl2 x 4 H2O | 2.50 | g | ||
|
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CoCl2 x 6 H2O | 0.50 | g | ||
|
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(NH4)6Mo7O24 x 4 H2O | 0.50 | g | ||
|
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FeSO4 x 7 H2O | 5.00 | g | ||
|
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CuSO4 x 5 H2O | 0.20 | g | ||
|
|
NaOH | 11.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH. | |||||
Seven vitamins solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
D-(+)-biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Calcium pantothenate | 100 | mg |
|
|
Pyridoxine hydrochloride | 300 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Distilled water | 1000 | ml |