1802
1802 .
BBM + V
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaNO3
(300 g/l stock solution) |
0.833 | ml | - | - | |
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
6.940 | ml | - | - | |
|
MgSO4 x 7 H2O
(7.5 g/l stock solution) |
10.000 | ml | - | - | |
|
K2HPO4 x 3 H2O
(4.35 g/l stock solution) |
17.240 | ml | - | - | |
|
KH2PO4
(17.5 g/l stock solution) |
10.000 | ml | 23.4 | 171.951 | |
|
NaCl
(250 g/l stock solution) |
0.100 | ml | - | - | |
| 1 Make up to 1000 ml with Milli Q water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 Cool down to room temperature. | |||||
| 4 Add the following components under sterile conditions: | |||||
| PIV metal solution | 6.000 | ml | - | - | |
| BBM + V Vitamine Mix | 150.000 | µl | - | - | |
Equipment needed: Filter
| 1 To 900 ml of Milli Q water, add 750 mg of Na2EDTA, and dissolve fully. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 750.00 | mg | 0.75 | 2.231 | |
| 2 Add the following salts in the order and amounts indicated: | |||||
|
FeCl3 x 6 H2O
(3.15 g/l stock solution) |
30.97 | ml | - | - | |
|
MnCl2 x 4 H2O
(4.1 g/l stock solution) |
10.25 | ml | - | - | |
|
ZnCl2
(1.2 g/l stock solution) |
4.16 | ml | - | - | |
|
CoCl2 x 6 H2O
(6 g/l stock solution) |
0.33 | ml | - | - | |
|
Na2MoO4 x 2 H2O
(0.2 g/l stock solution) |
20.00 | ml | - | - | |
| 3 Adjust pH to 6.4. | |||||
| 4 Make up to 1000 ml with Milli Q water. | |||||
| 5 Filtrate sterile (pore size 0.22 µm) and aliquot. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Thiamine
(1 .0 g/100 ml stock solution) |
10.00 | ml | - | - | |
|
Biotine
(0.5 mg/100 ml stock solution) |
10.00 | ml | - | - | |
|
Vitamine B12
(0.02 mg/ml stock solution) |
0.75 | ml | - | - | |
| 1 Make up to 30 ml with Milli Q water. | |||||
| 2 Filtrate sterile (pore size 0.22 µm) and aliquot. | |||||
Metadata
| Last modified: | 29.05.24 |
| Source: | MediaDive: DSMZ |
| Medium type: | Defined medium |
| Final pH: | 6.4 |
| Equipment needed: | Autoclave, Filter |
|
Description:
Bold´s Basal Medium with Vitamins from Bischoff & Bold 1963, modified after Starr and Zeikus 1993 |
|
| Edit in Medium builder | |