204 .
ASPERGILLUS NIDULANS MINIMAL MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500 | ml | ||
|
|
Solution B | 250 | ml | ||
|
|
Solution C | 200 | ml | ||
| 1 Autoclave separately for 15 min. Mix A, B and C before pouring plates. | |||||
Solution A #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaNO3 | 6.00 | g | ||
|
|
KH2PO4 | 1.52 | g | ||
|
|
KCl | 1.52 | g | ||
|
|
Distilled water | 500.00 | ml | ||
| 1 Adjust pH to 6.5 with 2 N NaOH. | |||||
Solution B #
250
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgSO4 x 7 H2O | 0.52 | g |
|
|
FeSO4 x 7 H2O | trace | |
|
|
ZnSO4 x 7 H2O | trace | |
|
|
Agar | 15.00 | g |
|
|
Distilled water | 250.00 | ml |