230 .
TRYPTONE YEAST EXTRACT MINERAL MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.2
Description:
Originally designed for Slackia; Pre-reduced, anaerobic.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone | 10.0 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
K2HPO4 | 0.5 | g | ||
|
|
KH2PO4 | 0.5 | g | ||
|
|
NaCl | 1.0 | g | ||
|
|
MgSO4 | 0.1 | g | ||
|
|
CaCl2 | 0.1 | g | ||
|
|
NaHCO3 | 6.0 | g | ||
|
|
Resazurin | 0.1 | mg | ||
|
|
L-Cysteine HCl x H2O | 0.3 | g | ||
|
|
Arginine | 3.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.2. | |||||