243 .
SEAWATER YEAST PEPTONE AGAR
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Selected strain: Marinobacterium jannaschii DSM 6295
This strain uses the standard medium without modifications.
Growth conditions: 25 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Yeast extract | 3 | g | ||
|
|
Agar | 12 | g | ||
|
|
Distilled water | 250 | ml | ||
|
|
Sea water
(filtered, aged) |
750 | ml | ||
| 1 Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Artificial sea water #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 28.13 | g |
|
|
KCl | 0.77 | g |
|
|
CaCl2 x 2 H2O | 1.60 | g |
|
|
MgCl2 x 6 H2O | 4.80 | g |
|
|
NaHCO3 | 0.11 | g |
|
|
MgSO4 x 7 H2O | 3.50 | g |
|
|
Distilled water | 1000.00 | ml |