253 .
MEDIUM FOR ECTOTHIORHODOSPIRA
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Equipment needed:
Autoclave
Filter
Final pH:
8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
NH4Cl | 0.80 | g | ||
|
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MgCl2 x 6 H2O | 0.10 | g | ||
|
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NaCl | 180.00 | g | ||
|
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Na2SO4 | 20.00 | g | ||
|
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Na2CO3 | 6.00 | g | ||
|
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Trace element solution (A) | 1.00 | ml | ||
|
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Yeast extract | 0.50 | g | ||
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Sodium succinate | 1.00 | g | ||
|
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Distilled water | 800.00 | ml | ||
| 1 Adjust pH to 8.5. | |||||
| 2 Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. After sterilization add 1 ml/l filter-sterilized vitamin solution (B), 200 ml of filter-sterilized solution Bicarbonate solution (C) and 10 ml/l sterilised Na2S x 9H2O solution (D). pH 8.5 | |||||
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Vitamin solution (B) | 1.00 | ml | ||
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Bicarbonate solution (C) | 200.00 | ml | ||
|
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Na2S x 9H2O solution (D) | 10.00 | ml | ||
Trace element solution (A) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeCl2 x 4 H2O | 1.8 | g | ||
|
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CoCl2 x 6 H2O | 250.0 | mg | ||
|
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NiCl2 x 6 H2O | 10.0 | mg | ||
|
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CuCl2 x 2 H2O | 10.0 | mg | ||
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MnCl2 x 4 H2O | 70.0 | mg | ||
|
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ZnCl2 | 100.0 | mg | ||
|
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H3BO3 | 500.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 30.0 | mg | ||
|
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Na2SeO3 x 5 H2O | 10.0 | mg | ||
|
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Distilled water | 1000.0 | ml | ||
| 1 For dissolving adjust pH to about 3 with 1 N HCl. | |||||
Vitamin solution (B) #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 10 | mg |
|
|
Nicotinamide | 35 | mg |
|
|
Thiamine-HCl x 2 H2O | 30 | mg |
|
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p-Aminobenzoic acid | 20 | mg |
|
|
Pyridoxal hydrochloride | 10 | mg |
|
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Calcium pantothenate | 10 | mg |
|
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Vitamin B12 | 5 | mg |
|
|
Distilled water | 100 | ml |