269 .
ACIDIPHILIUM MEDIUM
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* Medium for Acidocella aminolytica DSM 11237
Reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Final pH:
* 3.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 2.0 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
K2HPO4 | 0.5 | g | ||
|
|
MgSO4 x 7 H2O | 0.5 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
D-Glucose | 1.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4. | |||||