301 .
FALCIVIBRIO MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Wilkins-Chalgren-Anaerobe-Broth
(Oxoid) |
1000.0 | ml | ||
|
|
L-Cysteine | 0.3 | g | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Sterile serum
(horse, bovine or lamb) |
50.0 | ml | ||
| 1 Wilkins-Chalgren-Anaerobe-Broth (Oxoid) is supplemented with 0.3 g/l of L-cysteine and 1.0 mg/l of resazurin. Prepare the medium under 100% nitrogen gas. To the autoclaved medium add 50 ml/l of sterile serum (horse, bovine or lamb). | |||||