357 .
FLEXIBACTER CANADENSIS MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 0.1 | g | ||
|
|
KNO3 | 0.1 | g | ||
|
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
|
Sodium glycerophosphate | 0.1 | g | ||
|
|
Trace element solution SL-10 | 1.0 | ml | ||
|
|
Tris | 1.0 | g | ||
|
|
Thiamine-HCl x 2 H2O | 1.0 | mg | ||
|
|
Casamino acids | 1.0 | g | ||
|
|
Glucose
(sterilized separately) |
1.0 | g | ||
|
|
Vitamin B12 | 1.0 | µg | ||
|
|
Agar | 10.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.5. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||