389 .
ANNWOODIA MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 100 | ml | ||
|
|
Solution B | 870 | ml | ||
|
|
Solution C | 10 | ml | ||
|
|
Solution D | 20 | ml | ||
| 1 Autoclave solutions A, B and C and filter sterilize solution D. To complete the medium combine sterile solutions when cool. | |||||
Solution A #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 7.9 | g | ||
|
|
KH2PO4 | 1.5 | g | ||
|
|
Distilled water | 100.0 | ml | ||
| 1 Adjust to pH 7.6. | |||||
Solution B #
870
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4Cl | 0.4 | g |
|
|
MgSO4 x 7 H2O | 0.1 | g |
|
|
Agar , for solid medium | 15.0 | g |
|
|
Distilled water | 870.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 11.00 | g | ||
|
|
CaCl2 x 2 H2O | 7.34 | g | ||
|
|
MnCl2 x 4 H2O | 2.50 | g | ||
|
|
CoCl2 x 6 H2O | 0.50 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 5.00 | g | ||
|
|
CuSO4 x 5 H2O | 0.20 | g | ||
|
|
NaOH | 11.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH. | |||||