405 .
SP2 MEDIUM
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Selected strain: Bacillus sp. DSM 5310
This strain uses the standard medium without modifications.
Growth conditions: 60 °C
Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casitone
(Difco) |
1.00 | g | ||
|
|
Yeast extract
(Difco) |
0.20 | g | ||
|
|
Na-acetate | 0.02 | g | ||
|
|
Agar
, if required
(Difco) |
15.00 | g | ||
|
|
Artificial sea water | 1000.00 | ml | ||
| 1 Adjust pH to 7.2. | |||||
| 2 If natural sea water is used, the pH need not be adjusted. | |||||
Artificial sea water #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 24.7 | g | ||
|
|
KCl | 0.7 | g | ||
|
|
MgSO4 x 7 H2O | 6.3 | g | ||
|
|
MgCl2 x 6 H2O | 4.6 | g | ||
|
|
CaCl2 x 2 H2O | 1.3 | g | ||
|
|
NaHCO3 | 0.2 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water. | |||||