This strain uses the standard medium without modifications.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Casitone
(Difco) |
1.00 | g | ||
|
Yeast extract
(Difco) |
0.20 | g | ||
|
Na-acetate | 0.02 | g | ||
|
Agar
, if required
(Difco) |
15.00 | g | ||
|
Artificial sea water | 1000.00 | ml | ||
1 Adjust pH to 7.2. | |||||
2 If natural sea water is used, the pH need not be adjusted. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NaCl | 24.7 | g | ||
|
KCl | 0.7 | g | ||
|
MgSO4 x 7 H2O | 6.3 | g | ||
|
MgCl2 x 6 H2O | 4.6 | g | ||
|
CaCl2 x 2 H2O | 1.3 | g | ||
|
NaHCO3 | 0.2 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water. |