412 .
ACETOMICROBIUM FAECALIS MEDIUM
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Trypticase peptone | 2.000 | g | ||
|
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Yeast extract | 2.000 | g | ||
|
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Glucose | 4.000 | g | ||
|
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K2HPO4 | 0.225 | g | ||
|
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KH2PO4 | 0.255 | g | ||
|
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(NH4)2SO4 | 0.255 | g | ||
|
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NaCl | 0.500 | g | ||
|
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MgSO4 x 7 H2O | 0.100 | g | ||
|
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CaCl2 x 2 H2O | 0.070 | g | ||
|
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Na-acetate | 5.000 | g | ||
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Wolin's vitamin solution | 10.000 | ml | ||
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Modified Wolin's mineral solution | 10.000 | ml | ||
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Resazurin | 1.000 | mg | ||
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NaHCO3 | 6.000 | g | ||
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L-Cysteine HCl x H2O | 0.500 | g | ||
|
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Distilled water | 1000.000 | ml | ||
| 1 Autoclave under 2 bar N2:CO2 (80:20). | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium D-(+)-pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
|
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(DL)-alpha-Lipoic acid | 5.0 | mg |
|
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Distilled water | 1000.0 | ml |
Modified Wolin's mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
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MgSO4 x 7 H2O | 3.00 | g | ||
|
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MnSO4 x H2O | 0.50 | g | ||
|
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NaCl | 1.00 | g | ||
|
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FeSO4 x 7 H2O | 0.10 | g | ||
|
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CoSO4 x 7 H2O | 0.18 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
|
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ZnSO4 x 7 H2O | 0.18 | g | ||
|
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CuSO4 x 5 H2O | 0.01 | g | ||
|
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AlK(SO4)2 x 12 H2O | 0.02 | g | ||
|
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H3BO3 | 0.01 | g | ||
|
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Na2MoO4 x 2 H2O | 0.01 | g | ||
|
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NiCl2 x 6 H2O | 0.03 | g | ||
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Na2SeO3 x 5 H2O | 0.30 | mg | ||
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Na2WO4 x 2 H2O | 0.40 | mg | ||
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Distilled water | 1000.00 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||