414 .
ACIDAMINOCOCCUS FERMENTANS MEDIUM
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Selected strain: Acidaminococcus fermentans DSM 20731
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.0 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone | 5.0 | g | ||
|
|
Casamino acids | 10.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
Glucose | 5.0 | g | ||
|
|
Na glutamate | 4.0 | g | ||
|
|
Arginine | 1.0 | g | ||
|
|
Glycine | 1.0 | g | ||
|
|
DL-Tryptophan | 0.1 | g | ||
|
|
KH2PO4 | 2.0 | g | ||
|
|
Tween 80 | 0.5 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
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Distilled water | 1000.0 | ml | ||
| 1 pH 7.0 - 7.2. | |||||
| 2 Prepare and distribute medium anaerobically using the Hungate technique | |||||