420 .
BLOOD AGAR I
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Blood agar No 2 | 1000 | ml | ||
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Horse blood | 40 | ml/l | ||
| 1 Sterilize blood agar No 2 (Oxoid). After autoclaving cool to 50°C, then add 40 ml/l of sterile horse blood. | |||||
| 2 Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 1. For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). | |||||
Main sol. 1 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Peptone | 5 | g | ||
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Meat extract | 3 | g | ||
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Agar , for solid medium | 15 | g | ||
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Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Main sol. 220 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 15 | g | ||
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Soy peptone | 5 | g | ||
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NaCl | 5 | g | ||
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Agar | 15 | g | ||
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Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||