DSMZ 429b . CHOCOLATE AGAR

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Equipment needed: Autoclave

Final pH: n.d.

Select a strain (optional):

ml
Compound Amount Unit
Columbia agar base 1000 ml
Horse blood 10 %
1 Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80°C for 10 min. Mix well and pour plates after cooling to 50°C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1.

Main sol. 1 #

1000
Compound Amount Unit
Peptone 5 g
Meat extract 3 g
Agar , for solid medium 15 g
Distilled water 1000 ml
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Main sol. 220 #

1000
Compound Amount Unit
Casein peptone 15 g
Soy peptone 5 g
NaCl 5 g
Agar 15 g
Distilled water 1000 ml
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).

Main sol. 215 #

1000
Compound Amount Unit
Brain heart infusion
(Difco)
37 g
Distilled water 1000 ml