430 .
ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM
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Equipment needed:
Filter
Final pH:
8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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KH2PO4 | 0.80 | g | ||
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NH4Cl | 0.80 | g | ||
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CaCl2 x 2 H2O | 0.05 | g | ||
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MgCl2 x 6 H2O | 0.10 | g | ||
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NaCl | 120.00 | g | ||
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Na2SO4 | 15.00 | g | ||
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NaHCO3 | 10.00 | g | ||
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Na2CO3 , adjust if required | 5.00 | g | ||
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Vitamin solution (VA) | 1.00 | ml | ||
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Trace element solution (SLA) | 1.00 | ml | ||
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Na2S x 9 H2O | 0.50 | g | ||
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Na-acetate | 1.00 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 8.5, filter-sterilize and add appropriate amount of a sterile Na2S solution. | |||||
Trace element solution (SLA) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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FeCl2 x 4 H2O | 1.8 | g | ||
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CoCl2 x 6 H2O | 250.0 | mg | ||
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NiCl2 x 6 H2O | 10.0 | mg | ||
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CuCl2 x 2 H2O | 10.0 | mg | ||
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MnCl2 x 4 H2O | 70.0 | mg | ||
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ZnCl2 | 100.0 | mg | ||
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H3BO3 | 500.0 | mg | ||
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Na2MoO4 x 2 H2O | 30.0 | mg | ||
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Na2SeO3 x 5 H2O | 10.0 | mg | ||
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Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 2.0 - 3.0. | |||||
Vitamin solution (VA) #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 10 | mg |
|
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Nicotinic acid amide | 35 | mg |
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Thiamine-HCl x 2 H2O | 30 | mg |
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p-Aminobenzoic acid | 20 | mg |
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Pyridoxal hydrochloride | 10 | mg |
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Calcium pantothenate | 10 | mg |
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Vitamin B12 | 5 | mg |
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Distilled water | 100 | ml |