452 .
GLUCOSE MEDIUM (NAKAYAMA)
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Selected strain: Bacillus sp. DSM 6509
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Autoclave
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 10 | g | ||
|
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Peptone | 10 | g | ||
|
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Yeast extract | 15 | g | ||
|
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Agar | 10 | g | ||
|
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Distilled water | 980 | ml | ||
|
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Solution A | 10 | ml | ||
|
|
Solution B | 10 | ml | ||
|
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Solution C | 1 | ml | ||
| 1 Autoclave all solutions separately, final pH is 6.8. | |||||
Solution B #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgSO4 x 7 H2O | 3.00 | g |
|
|
NaCl | 0.10 | g |
|
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MnSO4 x 5 H2O | 0.10 | g |
|
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CuSO4 x 5 H2O | 0.01 | g |
|
|
Distilled water | 100.00 | ml |
Solution C #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 0.1 | g |
|
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Na3-citrate | 2.0 | g |
|
|
Distilled water | 100.0 | ml |