455 .
AMB MEDIUM
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Selected strain: Clostridium moniliforme DSM 3984
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Hungate tubes
Final pH:
6.9
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone | 4.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Starch
(soluble) |
1.00 | g | ||
|
|
Meat infusion | 25.00 | g | ||
|
|
Glucose | 5.00 | g | ||
|
|
Cysteine | 1.00 | g | ||
|
|
CaCl2 | 0.01 | g | ||
|
|
MgSO4 | 0.20 | g | ||
|
|
(NH4)2SO4 | 1.00 | g | ||
|
|
K2HPO4 | 15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH: 6.9. | |||||
| 2 Prepare and distribute medium anaerobically by using the Hungate technique. | |||||