465g .
MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE
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Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Na2HPO4 x 2 H2O | 3.5 | g | ||
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KH2PO4 | 1.0 | g | ||
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MgCl2 x 6 H2O | 0.1 | g | ||
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Trace element solution SL-4 | 1.0 | ml | ||
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Distilled water | 1000.0 | ml | ||
| 1 Prepare and autoclave medium. Add 5.5 g/l glycerol from a sterile solution. Add 645 mg/l cyanuric acid from a neutralized and filter sterilized stock solution and 10 ml vitamin solution (see medium 461) to the medium. | |||||
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Glycerol | 5.5 | g/l | ||
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Cyanuric acid | 645.0 | mg/l | ||
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Vitamin solution | 10.0 | ml | ||
| 2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Vitamin B12 | 50 | mg | ||
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Pantothenic acid | 50 | mg | ||
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Riboflavin | 50 | mg | ||
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Pyridoxamine hydrochloride | 10 | mg | ||
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Biotin | 20 | mg | ||
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Folic acid | 20 | mg | ||
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Nicotinic acid | 25 | mg | ||
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Nicotine amide | 25 | mg | ||
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alpha-lipoic acid | 50 | mg | ||
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p-Aminobenzoic acid | 50 | mg | ||
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Thiamine-HCl x 2 H2O | 50 | mg | ||
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Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Na2-EDTA | 0.50 | g | ||
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FeSO4 x 7 H2O | 0.20 | g | ||
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ZnSO4 x 7 H2O | 0.10 | g | ||
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MnCl2 x 4 H2O | 0.03 | g | ||
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H3BO3 | 0.30 | g | ||
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CoCl2 x 6 H2O | 0.20 | g | ||
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CuCl2 x 2 H2O | 0.01 | g | ||
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NiCl2 x 6 H2O | 0.02 | g | ||
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Na2MoO4 x 2 H2O | 0.03 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||