466 .
TSBY SALT MEDIUM
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Equipment needed:
Autoclave
Wheaton tubes
Final pH:
7.2
Description:
For cultivation of Carnobacterium sp.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase soy broth | 30.00 | g | ||
|
|
Yeast extract | 3.00 | g | ||
|
|
NaCl | 13.00 | g | ||
|
|
KCl | 0.34 | g | ||
|
|
MgCl2 x 6 H2O | 4.00 | g | ||
|
|
MgSO4 x 7 H2O | 3.45 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
CaCl2 x 2 H2O | 0.14 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.2 | |||||
| 2 Incubate under microaerophilic conditions | |||||