484 .
THIOMICROSPIRA THYASIRAE MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
7.3 - 7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 100 | ml | ||
|
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Solution B | 860 | ml | ||
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Solution C | 10 | ml | ||
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Solution D | 20 | ml | ||
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Solution E | 10 | ml | ||
| 1 Autoclave solutions A, B and C separately and mix when cool. After autoclaving, add thiosulfate, trace elements and vitamins from sterile stock solutions to complete the medium. Adjust pH of complete medium to 7.3 - 7.6. | |||||
Solution A #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 7.9 | g | ||
|
|
KH2PO4 | 1.5 | g | ||
|
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Distilled water | 100.0 | ml | ||
| 1 Adjust to pH 7.6. | |||||
Solution B #
860
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4Cl | 0.4 | g |
|
|
MgSO4 x 7 H2O | 0.1 | g |
|
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NaCl | 25.0 | g |
|
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Agar , for solid medium | 15.0 | g |
|
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Distilled water | 860.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Thiamine-HCl x 2 H2O | 10 | mg | ||
|
|
Nicotinic acid | 20 | mg | ||
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Pyridoxine hydrochloride | 20 | mg | ||
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p-Aminobenzoic acid | 10 | mg | ||
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Riboflavin | 20 | mg | ||
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Calcium pantothenate | 20 | mg | ||
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Biotin | 1 | mg | ||
|
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Vitamin B12 | 1 | mg | ||
|
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Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
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ZnSO4 x 7 H2O | 11.00 | g | ||
|
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CaCl2 x 2 H2O | 7.34 | g | ||
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MnCl2 x 4 H2O | 2.50 | g | ||
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CoCl2 x 6 H2O | 0.50 | g | ||
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(NH4)6Mo7O24 x 4 H2O | 0.50 | g | ||
|
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FeSO4 x 7 H2O | 5.00 | g | ||
|
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CuSO4 x 5 H2O | 0.20 | g | ||
|
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NaOH | 11.00 | g | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH. | |||||