514b .
MEDIUM 514 plus additional salt
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Selected strain: Halomonas halodenitrificans DSM 735
This strain uses the standard medium without modifications.
Growth conditions: 1-2 days
aerobic
28 °C
Equipment needed:
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 514 | 1000.0 | ml | ||
|
|
Agar
, for solid medium
(adjust in required in range 15-20) |
17.5 | g/l | ||
| 1 Use medium 514 to which is added NaCl at the concentration indicated (i.e. "514 + 6% NaCl" indicates use medium 514 to which 60 g/l NaCl has been added; see strain dependant modifications). | |||||
| 2 Final pH 7.6. The medium may be solidified by adding 15 - 20 g/l agar. | |||||
Main sol. 514 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto peptone | 5.00 | g | ||
|
|
Yeast extract
(Bacto ) |
1.00 | g | ||
|
|
Fe(III) citrate | 0.10 | g | ||
|
|
NaCl | 19.45 | g | ||
|
|
MgCl2
(anhydrous) |
5.90 | g | ||
|
|
Na2SO4 | 3.24 | g | ||
|
|
CaCl2 | 1.80 | g | ||
|
|
KCl | 0.55 | g | ||
|
|
NaHCO3 | 0.16 | g | ||
|
|
KBr | 0.08 | g | ||
|
|
SrCl2 | 34.00 | mg | ||
|
|
H3BO3 | 22.00 | mg | ||
|
|
Na-silicate | 4.00 | mg | ||
|
|
NaF | 2.40 | mg | ||
|
|
(NH4)NO3 | 1.60 | mg | ||
|
|
Na2HPO4 | 8.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water. | |||||