514f .
MARINE BROTH (ROTH CP73)
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* Medium for Photobacterium carnosum DSM 105454
Mix 1 volume medium 514 and one volume medium 535 (trypticase soy agar/broth)
Growth conditions: 15 °C
Equipment needed:
Final pH:
7.4 - 7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5.00 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
Fe(III) citrate | 0.10 | g | ||
|
|
NaCl | 19.40 | g | ||
|
|
MgCl2 | 8.80 | g | ||
|
|
Na2SO4 | 3.24 | g | ||
|
|
CaCl2 | 1.80 | g | ||
|
|
KCl | 0.55 | g | ||
|
|
NaHCO3 | 0.16 | g | ||
|
|
KBr | 0.08 | g | ||
|
|
SrCl2 | 34.00 | mg | ||
|
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H3BO3 | 22.00 | mg | ||
|
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Na-silicate | 4.00 | mg | ||
|
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NaF | 2.40 | mg | ||
|
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(NH4)NO3 | 1.60 | mg | ||
|
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Na2HPO4 | 8.00 | mg | ||
|
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Distilled water | 1000.00 | ml | ||
|
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Agar , if required | 15.00 | g/l | ||
|
|
545. TRYPTONE SOYA BROTH (TSB) | 1000.00 | ml | ||
| 1 Final pH should be 7.6 ± 0.2 at 25°C. Add 15.0 g/l agar for solid media. We recommend using the medium supplied by Carl Roth GmbH + Co. KG for Marinomonas mediterranea DSM 23531. | |||||
545. TRYPTONE SOYA BROTH (TSB) * #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D(+)-Glucose | 2.5 | g | ||
|
|
NaCl | 5.0 | g | ||
|
|
K2HPO4 | 2.5 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany). | |||||