DSMZ 535c . TRYPTICASE SOY BROTH AGAR

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Equipment needed: Autoclave

Final pH: n.d.

Select a strain (optional):

ml
Compound Amount Unit
Medium 535 1000 ml
Tween 80 1 %
1 Add 1 % Tween 80 to medium 535.

Main sol. 535 #

1000
Compound Amount Unit
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459)
30 g
Agar 20 g
Distilled water 1000 ml
1 pH 7.3
2 Autoclave at 121°C for 15 min.