pH 5.5
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Beef extract | 1 | g | ||
|
Glucose | 10 | g | ||
|
N-Z amine
(enzymatic digest of casein) |
2 | g | ||
|
Yeast extract | 1 | g | ||
|
Agar | 20 | g | ||
|
Distilled water | 1000 | ml | ||
1 Adjust pH to 7.3 with NaOH. | |||||
2 Modified Bennett's agar can also be used. In it, glucose is replaced by glycerol (10.0 g) and N-Z amine by Bacto casein (2.0 g, Difco). |