586 .
THAUERA AROMATICA MEDIUM
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* Medium for Thauera phenylacetica DSM 14743
PH 7.3; anaerobic or aerobic. For aerobic growth, omit nitrate
Growth conditions: 30 °C
Equipment needed:
Autoclave
Filter
Final pH:
* 7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500 | ml | ||
|
|
Solution B | 500 | ml | ||
|
|
Trace element solution SL-10 | 10 | ml | ||
|
|
Vitamin solution | 5 | ml | ||
| 1 Adjust solutions A and B to pH 7.2, autoclave separately and combine after cooling. Add 10 ml of sterile trace elements solution SL-10 (see below) and 5 ml vitamin solution (see below). | |||||
Solution B #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4Cl | 0.530 | g |
|
|
MgSO4 x 7 H2O | 0.200 | g |
|
|
KNO3 | 2.000 | g |
|
|
CaCl2 x 2 H2O | 0.025 | g |
|
|
Na-benzoate | 0.720 | g |
|
|
Distilled water | 500.000 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 50 | mg | ||
|
|
Pantothenic acid | 50 | mg | ||
|
|
Riboflavin | 50 | mg | ||
|
|
Pyridoxamine hydrochloride | 10 | mg | ||
|
|
Biotin | 20 | mg | ||
|
|
Folic acid | 20 | mg | ||
|
|
Nicotinic acid | 25 | mg | ||
|
|
Nicotine amide | 25 | mg | ||
|
|
alpha-lipoic acid | 50 | mg | ||
|
|
p-Aminobenzoic acid | 50 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 50 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||