590 .
DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM
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Equipment needed:
Filter
Final pH:
7.0 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(Difco, Bacto ) |
1.0 | g | ||
|
|
Na2-DL-malate | 1.0 | g | ||
|
|
Hutner's salts | 20.0 | ml | ||
|
|
Artificial sea water, 3 times concentrated | 980.0 | ml | ||
|
|
Agar , for solid medium | 18.0 | g/l | ||
|
|
NaHCO3
(8 %) |
28.4 | ml | ||
| 1 pH 7.0-7.2 | |||||
| 2 Agar at a concentration of 18 g/l is added for solid media. | |||||
| 3 Prepare an 8% solution of NaHCO3 in half full 100ml serum bottles closed with a rubber septum and autoclave. Add 28.4 ml of the sterile 8% solution of NaHCO3 (2.822g/l) per litre of the sterile, cooled medium. | |||||
Artificial sea water, 3 times concentrated #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 70.43 | g | ||
|
|
Na2SO4 | 11.75 | g | ||
|
|
NaHCO3 | 2.88 | g | ||
|
|
KCl | 1.99 | g | ||
|
|
KBr | 0.29 | g | ||
|
|
MgCl2 x 6 H2O | 31.86 | g | ||
|
|
CaCl2 x 2 H2O | 4.35 | g | ||
|
|
H3BO3 | 0.08 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled. | |||||
Hutner's salts #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | ||
|
|
MgSO4 x 7 H2O | 29.700 | g | ||
|
|
CaCl2 x 2 H2O | 3.335 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | ||
|
|
FeSO4 x 7 H2O | 99.000 | mg | ||
|
|
"Metals 44" | 50.000 | ml | ||
|
|
Distilled water | 950.000 | ml | ||
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
"Metals 44" #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na-EDTA | 250.0 | mg | ||
|
|
ZnSO4 x 7 H2O | 1095.0 | mg | ||
|
|
FeSO4 x 7 H2O | 500.0 | mg | ||
|
|
MnSO4 x H2O | 154.0 | mg | ||
|
|
CuSO4 x 5 H2O | 39.2 | mg | ||
|
|
Co(NO3)2 x 6 H2O | 24.8 | mg | ||
|
|
Na2B4O7 x 10 H2O | 17.7 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||