598 .
HALOVIBRIO VARIABILIS MEDIUM
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Selected strain: Halomonas utahensis DSM 3051
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 95.0 | g | ||
|
|
MgSO4 x 7 H2O | 81.0 | g | ||
|
|
KCl | 1.0 | g | ||
|
|
Yeast extract | 7.5 | g | ||
|
|
Proteose peptone | 2.5 | g | ||
|
|
Trace element solution SL-4 | 10.0 | ml | ||
|
|
Vitamin solution | 10.0 | ml | ||
|
|
Distilled water | 980.0 | ml | ||
| 1 pH 7.5 | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 0.20 | mg |
|
|
Folic acid | 0.20 | mg |
|
|
Pyridoxine hydrochloride | 1.00 | mg |
|
|
Thiamine-HCl x 2 H2O | 0.50 | mg |
|
|
Riboflavin | 0.50 | mg |
|
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Nicotinic acid | 0.50 | mg |
|
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Pantothenic acid | 0.50 | mg |
|
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Vitamin B12 | 0.01 | mg |
|
|
p-Aminobenzoic acid | 0.50 | mg |
|
|
Lipoic acid | 0.50 | mg |
|
|
Distilled water | 1000.00 | ml |
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
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ZnSO4 x 7 H2O | 0.10 | g | ||
|
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MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
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CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||