600a .
M13a (PIRELLULA) MEDIUM
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Peptone | 0.25 | g | ||
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Yeast extract | 0.25 | g | ||
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Glucose | 0.25 | g | ||
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Vitamin solution | 10.00 | ml | ||
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Hutner's salts | 20.00 | ml | ||
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Tris-HCl buffer
(0.1 M, pH 7.5) |
50.00 | ml | ||
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Artifical sea water | 500.00 | ml | ||
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Distilled water | 420.00 | ml | ||
| 1 For agar plates increase the pH of the Tris/HCl buffer to 8.5. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Vitamin B12 | 0.2 | mg | ||
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Biotin | 4.0 | mg | ||
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Thiamine-HCl x 2 H2O | 10.0 | mg | ||
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Calcium pantothenate | 10.0 | mg | ||
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Folic acid | 1.0 | mg | ||
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Riboflavin | 10.0 | mg | ||
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Nicotinamide | 10.0 | mg | ||
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p-Aminobenzoic acid | 10.0 | mg | ||
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Pyridoxine hydrochloride | 10.0 | mg | ||
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Distilled water | 1000.0 | ml | ||
| 1 Filter-sterilized. | |||||
Artifical sea water #
1000
Hutner's salts #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Nitrilotriacetic acid
(NTA) |
10.000 | g | ||
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MgSO4 x 7 H2O | 29.700 | g | ||
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CaCl2 x 2 H2O | 3.335 | g | ||
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(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | ||
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FeSO4 x 7 H2O | 99.000 | mg | ||
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"Metals 44" | 50.000 | ml | ||
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Distilled water | 950.000 | ml | ||
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
"Metals 44" #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Na-EDTA | 250.0 | mg | ||
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ZnSO4 x 7 H2O | 1095.0 | mg | ||
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FeSO4 x 7 H2O | 500.0 | mg | ||
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MnSO4 x H2O | 154.0 | mg | ||
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CuSO4 x 5 H2O | 39.2 | mg | ||
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Co(NO3)2 x 6 H2O | 24.8 | mg | ||
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Na2B4O7 x 10 H2O | 17.7 | mg | ||
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Distilled water | 1000.0 | ml | ||
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||