619 .
HYPHOMICROBIUM METHYLOVORUM MEDIUM
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Selected strain: Hyphomicrobium methylovorum DSM 5458
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Methanol | 10.0 | ml | ||
|
|
(NH4)2HPO4 | 3.0 | g | ||
|
|
NaCl | 1.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
FeSO4 x 7 H2O | 10.0 | mg | ||
|
|
MnSO4 x 1 H2O | 5.0 | mg | ||
|
|
Vitamin mixture | 5.0 | ml | ||
|
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Agar | 15.0 | g | ||
|
|
Tap water | 1000.0 | ml | ||
| 1 Sterilize methanol by filtration, and add to other components sterilized by autoclaving. | |||||
Vitamin mixture #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 0.2 | mg |
|
|
Calcium pantothenate | 40.0 | mg |
|
|
Folic acid | 0.2 | mg |
|
|
Inositol | 200.0 | mg |
|
|
Niacin | 40.0 | mg |
|
|
p-Aminobenzoic acid | 20.0 | mg |
|
|
Pyridoxine hydrochloride | 40.0 | mg |
|
|
Riboflavin | 40.0 | mg |
|
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Thiamine-HCl x 2 H2O | 20.0 | mg |
|
|
Choline | 100.0 | mg |
|
|
Vitamin B12 | 2.0 | µg |
|
|
Distilled water | 1000.0 | ml |