623
623 .
THERMOCOCCUS LITORALIS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Thermococcus litoralis DSM 5474
This strain uses the standard medium without modifications.
Growth conditions: 83 °C
Main sol. 623 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto peptone | 5.000 | g | 5 | - | |
| Yeast extract | 1.000 | g | 1 | - | |
| NaCl | 19.450 | g | 19.45 | 332.82 | |
| MgCl2 x 6 H2O | 12.600 | g | 12.6 | 61.977 | |
| Na2SO4 | 3.420 | g | 3.42 | 24.078 | |
| CaCl2 x 2 H2O | 2.380 | g | 2.38 | 16.189 | |
| KCl | 0.550 | g | 0.55 | 7.378 | |
| Na2CO3 | 0.610 | g | 0.61 | 5.755 | |
| KBr | 0.080 | g | 0.08 | 0.672 | |
| SrCl2 x 6 H2O | 0.057 | g | 0.057 | 0.214 | |
| Boric acid | 0.022 | g | 0.022 | 0.356 | |
| Na-meta silicate | 4.000 | mg | 0.004 | - | |
| NaF | 2.400 | mg | 0.002 | 0.057 | |
| KNO3 | 1.600 | mg | 0.002 | 0.016 | |
| Na2HPO4 | 0.010 | g | 0.01 | 0.07 | |
| Sulfur | 10.000 | g | 10 | 311.818 | |
| Resazurin | 0.100 | g | 0.1 | 0.436 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Prepare the medium under 100% N2 and adjust final pH to 6.5. The medium, containing sulphur, is sterilized by steaming on three consecutive days for 3 h. Reduce the medium with a sterile Na2S stock solution to give a final concentration of 0.5 g/l. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Thermococcus litoralis DSM 5474
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.45 | 332.82 |
| MgCl2 x 6 H2O | 12.6 | 61.977 |
| Sulfur | 10 | 311.818 |
| Bacto peptone | 5 | - |
| Na2SO4 | 3.42 | 24.078 |
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