634b .
RCM MEDIUM (with Sea Salts)
What is this page?
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
read more
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
dehydrated RCM medium | 38.0 | g | ||
|
|
Sea salts
(SIGMA) |
30.0 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) and SIGMA Sea Salts (30.0 g/l) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.8. | |||||