634c .
RCM MEDIUM (N2/CO2)
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
dehydrated RCM medium | 38.0 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
Sodium carbonate | 1.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use, add 1.0 g of sodium carbonate per liter medium from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Check pH of medium after autoclaving and adjust pH to 6.8, if necessary. | |||||