673 .
THERMOCOCCUS PROFUNDUS MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Water bath
Final gas composition:
100 % N2
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Final pH:
7.2
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g | ||
|
|
KH2PO4 | 0.28 | g | ||
|
|
MgSO4 x 7 H2O | 0.25 | g | ||
|
|
CaCl2 x 2 H2O | 0.07 | g | ||
|
|
FeCl3 x 6 H2O | 0.02 | g | ||
|
|
MnCl2 x 4 H2O | 1.80 | mg | ||
|
|
Na2B4O7 x 10 H2O | 4.50 | mg | ||
|
|
ZnSO4 x 7 H2O | 0.22 | mg | ||
|
|
CuCl2 x 2 H2O | 0.05 | mg | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | mg | ||
|
|
VOSO4 x 2 H2O | 0.03 | mg | ||
|
|
CoSO4 x 7 H2O | 0.01 | mg | ||
|
|
NaCl | 25.00 | g | ||
|
|
Resazurin | 1.00 | mg | ||
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Yeast extract | 1.00 | g | ||
|
|
Sulfur
(powdered) |
5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Peptone | 5.00 | g | ||
| 1 Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere. | |||||
| 2 Adjust final pH to 7.2. | |||||